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. 2019 Dec 30;9:20299. doi: 10.1038/s41598-019-56869-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Exposure to chemical stressors altered the levels of lncRNA expression. Following treatment of HepG2 cells with 100 μM (a) hydrogen peroxide, (b) mercury II chloride, or (c) etoposide for 24 hours, RT-qPCR was performed to determine the expression levels of the indicated RNAs normalized relative to GAPDH, ACTB, HPRT1, and PGK1. All values are means ± SD from four independent experiments (*P < 0.05, Student’s t test). The expression levels of treated cells divided by those of untreated cells are shown on y-axis, so a value of 0 indicates that the RNA could not be detected, while a value of 1 indicates no change in expression level compared to untreated controls. Gray dotted lines indicated cut-off values.