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. Author manuscript; available in PMC: 2021 Feb 1.
Published in final edited form as: Biochim Biophys Acta Mol Cell Res. 2019 Nov 21;1867(2):118613. doi: 10.1016/j.bbamcr.2019.118613

Fig. 3.-. Laforin and malin co-localize with Beclin1.

Fig. 3.-

Representative images of U2OS cells transfected with the plasmids indicated on their left. The subcellular localization of the corresponding proteins was analyzed either by direct fluorescence (GFP and GFP-malin) or by immunofluorescence using anti-FLAG (coupled to an Alexa-Fluor 633 secondary antibody) and anti-Beclin1 (coupled to an Alexa-Fluor 568 secondary antibody) antibodies, as described in Materials and Methods. Fluorescence associated with the corresponding proteins was analyzed by confocal microscopy. DAPI staining was in blue. A merged image of the fluorescent dyes is also shown. Bar: 20 μm.