Skip to main content
. Author manuscript; available in PMC: 2021 Feb 1.
Published in final edited form as: Biochim Biophys Acta Mol Cell Res. 2019 Nov 21;1867(2):118613. doi: 10.1016/j.bbamcr.2019.118613

Fig. 5.-. The laforin/malin complex interacts with Vps34 and Vps15 and promotes their ubiquitination.

Fig. 5.-

A) CFP-laforin pull-downs Vps34 and Vps15 in the presence of malin. HEK293 cells were co-transfected with the indicated combination of plasmids expressing Myc-Beclin1, Myc-Vps34/V5-Vps15, FLAG-malin, CFP (empty) and CFP-laforin. Cells were lysed and 1.5 mg of proteins were analyzed as in the legend of Fig. 2A using the appropriated antibodies. B) Laforin/malin complex ubiquitinates Vps34 and Vps15. HEK293 cells were transfected with the indicated plasmids and ubiquitination of Vps34 and Vps15 was analyzed as in the legend of Fig. 4A. In A) and B), a representative blot of three independent experiments is shown. Molecular size markers are indicated in kDa on the right of the blots.