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. Author manuscript; available in PMC: 2021 Feb 1.
Published in final edited form as: Biochim Biophys Acta Mol Cell Res. 2019 Nov 21;1867(2):118613. doi: 10.1016/j.bbamcr.2019.118613

Fig. 7.-. The laforin/malin complex promotes the ubiquitination of ATG14L.

Fig. 7.-

A) CFP-laforin does not pull-down ATG14L in the presence of malin. HEK293 cells were co-transfected with the indicated combination of plasmids expressing FLAG-ATG14L, HA-malin, CFP (empty) and CFP-laforin. Cells were lysed and 1.5 mg of proteins were analyzed as in the legend of Fig. 2A using the appropriated antibodies. B) Laforin/malin complex ubiquitinates ATG14L. HEK293 cells were transfected with the indicated plasmids and ubiquitination of ATG14L was analyzed as in the legend of Fig. 4A. In A) and B), a representative blot of three independent experiments is shown. Molecular size markers are indicated in kDa on the right of the blots.