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. Author manuscript; available in PMC: 2021 Feb 1.
Published in final edited form as: Biochim Biophys Acta Mol Cell Res. 2019 Nov 21;1867(2):118613. doi: 10.1016/j.bbamcr.2019.118613

Fig. 9.-. The laforin/malin complex does not affect Bcl2, AMBRA1 or ULK1.

Fig. 9.-

A) CFP-laforin does not pull-down Bcl2 in the presence of malin. HEK293 cells were co-transfected with the indicated combination of plasmids expressing FLAG-Bcl2, HA-malin, and CFP-laforin. Cells were lysed and 1.5 mg of proteins were analyzed as in the legend of Fig. 2A using the appropriated antibodies. B) Laforin/malin complex does not ubiquitinate Bcl2. HEK293 cells were transfected with the indicated plasmids and ubiquitination of Bcl2 was analyzed as in the legend of Fig. 4A. C) and D) The laforin/malin complex does not ubiquitinate AMBRA1 or ULK1. HEK293 cells were transfected with the indicated plasmids and ubiquitination of AMBRA1 (C) and ULK1 (D) was analyzed as in the legend of Fig. 4A. In A) to D), a representative blot of three independent experiments is shown. Molecular size markers are indicated in kDa on the right of the blots.