Figure 3.

Hairy roots expressing GFP under control of the ZCT1 promoter respond to the auxin 1‐NAA and MJ. Hairy roots expressing GFP under the control of the ZCT1 promoter (pZCT1::GFP) or without GFP transgene (no GFP) were treated with varying concentrations of hormones for 24 hr. The mock treatment contains 0.1% ethanol. No ethanol and no hormones were added to the untreated samples. Each experiment consisted of at least three biological replicates per hormone dosage with a total of three independent assays performed (represented by +, ○, and Δ symbols). For each independent assay, the GFP signal (RFU/mg FW) of each sample is normalized to the average of the pZCT1::GFP mock control. Data were analyzed using a one‐way ANOVA, and significant differences, compared to the pZCT1::GFP mock control, were determined using the Dunnett's method. p‐values < .05 are indicated with one star (*), and p‐values < .001 are indicated with three stars (***). NAA, 1‐naphthalene acetic acid; GA, gibberellin (here GA₃); RFU, relative fluorescence units; FW, fresh weight