Figure 5.

The GA‐responsive complex (GARC) is a contributor to ZCT1 promoter activity under the EASI conditions. Catharanthus roseus seedlings, at three days after transfer to light, were vacuum‐infiltrated with Agrobacterium tumefaciens (OD₆₀₀ = 0.2) containing reporter constructs with various ZCT1 promoter deletion‐driven FLUC‐I reporter and the AtuNOS promoter‐driven RLUC‐I normalization reporter. Samples were taken three days postinfection. The relative promoter activity is the ratio of FLUC (firefly luciferase) to RLUC (Renilla luciferase) activity for each sample normalized to the ratio of FLUC to RLUC activity of the pZCT1_744 control (set to 1). The experiment was carried out in three independent assays (represented by +, ○, and Δ symbols). Each data point represents the luciferase activity of 2 seedlings. The vertical line of the boxes shows the median, the ends of the boxes show the 1st and 3rd quantile, and whiskers show the lowest and highest data point values within the 1st and 3rd quartile. Detailed information on the promoter sequence and identified cis regulative elements can be found in Figure S1. The data were log‐normal‐transformed to obtain normal distributed data. Data were analyzed using a one‐way ANOVA, and significant differences between groups were determined using the Tukey–Kramer method on log‐normal‐transformed data