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. Author manuscript; available in PMC: 2019 Dec 31.
Published in final edited form as: Methods Mol Biol. 2018;1688:99–109. doi: 10.1007/978-1-4939-7386-6_5

Fig. 3.

Fig. 3

SUV Preparation. Liposome preparations begin with a glass tube (A). Add lipids (B) and dissolve in chloroform (C). After depositing on glass, the lipids should be completely dried (D and inset). Rehydrate lipids with water (E), and vortex repeatedly to resuspend (F). After a freeze-thaw cycle, return to the glass tube for sonication; lipids should retain appearance. Sonicate until clear (H), (G) shows an example of incompletely sonicated liposomes, which retains opacity.