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. Author manuscript; available in PMC: 2019 Dec 31.
Published in final edited form as: Methods Mol Biol. 2018;1688:99–109. doi: 10.1007/978-1-4939-7386-6_5

Table 1:

SSLB preparation

Bead Size
(nm)		 Theoretical lipid
required to coat beads
(per mg beads)		 Experimentally
recommended amount		 Time and speed
needed to pellet
20 – 30 0.4 mg 1.6 mg 15 min at 200,000 x g
50 0.2 mg 0.8 mg 10 min at 16,000 x g
100 0.1 mg 0.4 mg 5 min at 16,000 x g
1000 0.01 mg 0.04 mg 2 min at 16,000 x g