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Galacturonate bound to the OtCE15A S267A variant (PDB code 6SZO). Presumably, the cleavage rate was too fast to capture a Michaelis–Menten complex with methyl galacturonoate over the crystal soaking period (60 s) but allowed capture of the galacturonate product in the active site. The galacturonate is shown in yellow sticks, and a DMSO molecule, used as a solvent for the substrate, present in the oxyanion hole is shown in sticks.
