Table 1.

Kinetic parameters of BnzGlcA esterase activity of the WT OtCE15A and alanine variants of its catalytic residues

Errors represent S.D. from triplicate experiments

OtCE15A	Km	kcat	kcat/Km
	mm	s−1	s−1 mm−1
WT	3.57 ± 0.091	16.6 ± 0.11	(4.65 ± 0.12) × 103
S267A	2.83 ± 0.66	0.000983 ± 0.000059	(3.47 ± 0.84) × 10−1
H408A	0.184 ± 0.035	0.00999 ± 0.00040	(5.43 ± 1.06) × 101
E290A	2.03 ± 0.11	10.3 ± 0.11	(5.07 ± 0.28) × 103
D356A	1.86 ± 0.11	5.21 ± 0.073	(2.80 ± 0.17) × 103
E290A/D356A	0.502 ± 0.034	0.196 ± 0.0025	(3.90 ± 0.27) × 102