Figure 3.

Effects of cTnC–I4M on Ca²⁺-activated tension at SL 2.1 μm in CMP. A, normalized steady-state isometric tension as a function of pCa recorded at 30 °C. Quantitative comparison of pCa₅₀ (B), n_Hill (C), and maximum steady-state isometric tension (D) values for CMP reconstituted with cTnC–WT or -I4M. The force values were normalized to the maximum force for each CMP. Data are shown as mean ± S.D. n = 9 CMP for cTnC–WT and n = 8 CMP for cTnC–I4M. Horizontal bars represent the mean, and * denotes statistical significance between the means (p < 0.05) determined by an unpaired Student's t-test.