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. 2019 Nov 13;294(52):19896–19906. doi: 10.1074/jbc.RA119.010379

Figure 2.

Figure 2.

Selective up-regulation of PD-1 expression on T hybridoma cells by GCs. A, surface PD-1 expression levels on DO11.10 T hybridoma cells after treatments with GCs. Cells were treated with the indicated GCs (100 nm) for 24 h, and their PD-1 expression level was analyzed by flow cytometry. B, expression levels of cell surface molecules on DO11.10 T hybridoma cells after dexamethasone treatment. Cells were treated with dexamethasone (100 nm) for 24 h, and the expression levels of the indicated molecules were analyzed by flow cytometry. C, expression of cell surface molecules on DO11.10 T hybridoma cells after antigenic stimulation in the presence of dexamethasone. DO11.10 T hybridoma cells were stimulated by co-culturing with pOVA323–339–pulsed IIA1.6 cells for 24 h in the presence dexamethasone (100 nm) and expression of the indicated molecules on live DO11.10 T hybridoma cells (PI−B220−TCR-β+) cells were analyzed by flow cytometry. Representative histogram plots and the mean ± S.D. of three independent experiments are shown (A–C). Gray-shaded histograms represent isotype control Ig staining (A–C). One-way ANOVA with Dunnett's multiple comparisons test (compared with DMSO-treated cells, A) and with Tukey's multiple comparisons test (B and C) was used. *, p < 0.05; **, p < 0.01; ***, p < 0.001.