Figure 2.

Experimental workflow. MPO was isolated from pooled neutrophils of five healthy donors by concanavalin A–Sepharose and size-exclusion chromatography (discovery pool) or only concanavalin A–Sepharose chromatography (validation pool). The resulting material was then analyzed by both a bottom-up (glyco)proteomics approach (left branch) and native MS (right branch). Confidence in the glycan assignment was achieved by comparing the weighted mass of the bottom-up results with the mass observed by native MS.