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Native mass spectrum of MPO reveals the exclusive presence of a MPO dimer with an average molecular weight (Mw) of 144,180 Da. A comparison of this Mw with the theoretical Mw of the dimer, based on the peptide backbone mass and all detected glycosylations, is provided in the inset. The average contribution to the mass of the glycosylation was determined from the bottom-up glycoproteomics experiments, overall leading to a high congruency between the theoretical and observed masses.
