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. 2019 Nov 22;294(52):20207–20221. doi: 10.1074/jbc.RA119.010650

Figure 6.

Figure 6.

IAV protein PA-X down-regulates HDAC4 at mRNA level. A, A549 cells were transfected with the control siRNA or viral PA siRNA and subsequently infected with PR8 at a m.o.i. of 1.0 for 24 h. Then, the levels of HDAC4 mRNA, actin mRNA, and 18S RNA were detected by qPCR. The levels of HDAC4 mRNA in all samples were normalized with corresponding actin mRNA or 18S RNA levels. Finally, the normalized level of HDAC4 mRNA in control siRNA-transfected/uninfected (CT/UNI) cells were considered 100% to compare its levels in the control siRNA-transfected/infected (CT/INF) cells and PA siRNA-transfected/infected (PA/INF) cells. B, A549 cells were transfected with empty plasmid pcDNA3 (PD) or PA-X plasmid (PX) as above, and the levels of HDAC4 mRNA, actin mRNA, and 18S RNA were detected by qPCR. Then, the levels of HDAC4 mRNA in all samples were normalized with corresponding actin mRNA or 18S RNA levels. Finally, the normalized level of HDAC4 mRNA in pcDNA3-transfected cells (PD) were considered 100% to compare its level in the PA-X plasmid-transfected cells (PX). Error bars represent the mean ± S.E. of three independent experiments. The asterisks represent p values mentioned in the text calculated by ANOVA, and indicate the significant differences in means. MW, molecular weight.