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. 2019 Nov 18;294(52):20185–20195. doi: 10.1074/jbc.RA119.010257

Figure 1.

Figure 1.

Y59H polymorphism affects homodimer formation. A, C, and E, C1R-B*27:03, C1R-B*27:05, C1R-B*27:09, C1R-B*27:07, C1R-B*27:07-Y59H, C1R-B*27:08, and C1R-B*27:08-Y59H cell lysates were separated by 12% SDS-PAGE and analyzed by immunoblotting using the HC10 antibody and an antibody recognizing GAPDH as loading control. Representative blots are shown. The addition of H2O2 to the cells is indicated above the lanes. Red arrow, HLA-B27 homodimers; blue arrow, monomeric forms. The addition of H2O2 to the cells as well as the presence of DTT in the SDS-PAGE loading buffer is indicated above the lanes. B, D, F, background-corrected densitometric analyses showing the ratio of HLA-B27 homodimer to monomer. n = 4; mean ± S.E. (error bars). p value was calculated using the Welch's t test (*, p < 0.05; **, p < 0.001; NS, nonsignificant).