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Ellman's assay. The diagram shows the reaction rate of KK10-refolded HLA-B*27:03 (orange) and HLA-B*27:05 (pink) with DTNB (Ellman's reagent). The production of the 2-nitro-5-thiobenzoate dianion was measured colorimetrically for 1 h at an absorbance of 412 nm. n = 3; a representative result is shown.
