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. 2019 Nov 18;294(52):19988–19996. doi: 10.1074/jbc.RA119.010821

Figure 4.

Figure 4.

Insulin-stimulated GLUT4 exocytosis is inhibited in Exoc7 KO adipocytes. A, normalized surface levels of the GLUT4 reporter in WT and Exoc7 KO adipocytes. After treatment with 100 nm insulin for the indicated periods, surface levels of the GLUT4 reporter were measured using flow cytometry as described in Fig. 1E. Data are presented as mean ± S.D.; n = 3. B, initial exocytosis rates of the GLUT4 reporter in WT and Exoc7 KO adipocytes treated with insulin. The initial exocytosis rates were calculated based on increases in surface levels of the GLUT4 reporter during the first 10 min of insulin treatment shown in A. Datasets are normalized to untreated WT cells. Data are presented as mean ± S.D. n = 3. ***, p < 0.001. C, dose dependence of insulin-stimulated translocation of the GLUT4 reporter in WT and Exoc7 KO adipocytes. Cells were treated with the indicated concentrations of insulin for 30 min before the surface levels of the reporter were measured by flow cytometry. NT, not treated. Datasets are normalized to untreated WT cells. Data are presented as mean ± S.D.; n = 3.