Figure 6.

Tight regulation of adipocyte genome editing using the inducible CRISPR system. A, representative immunoblots showing expression of the indicated proteins in WT and Exoc7-targeted adipocytes with or without dox induction. B, representative immunoblots showing expression of the indicated proteins in Exoc7 KO adipocytes with or without expression of a human EXOC7 rescue gene. For insulin stimulation, cells were treated with 10 nm insulin for 10 min. C, normalized surface levels of the GLUT4 reporter in WT and Exoc7 KO adipocytes with or without expression of the human EXOC7 rescue gene. The cells were left untreated or treated with 100 nm insulin for 30 min. Data are presented as mean ± S.D.; n = 3. ***, p < 0.001; n.s., not significant, p > 0.05.