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Influence of the +1 position of RNA on phosphate removal by YvcI. A, ppp-RNA (XGU 5S rRNA, 5′-labeled (*), 0.5 μm) was incubated with YvcI (2 μm) in 1× manganese buffer. 8% PAGE, phosphorimaging of radioactivity is shown. B, quantification of the ppp-RNA from A, showing means ± S.D. of three independent experiments (technical replicates).
