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Influence of RNA secondary structure on YvcI's RNA pyrophosphohydrolase activity. A and D, schemes of 5′-ppp-5S rRNA with different 5′- and 3′-termini initiating either with G (A) or A (D). B and E, ppp-RNA (ppp-5S rRNA, 5′-labeled, 0.5 μm) was incubated with YvcI (2 μm) in 1× manganese buffer. PEI-cellulose TLC, phosphorimaging of radioactivity is shown. C and F, quantification of the ppp-RNA from B and E, showing means ± S.D. of three independent experiments (technical replicates).
