De novo activation of Bmp10 preserves cardiac function.
A, Sirus red/fast green staining showing a significantly reduced level of collagen deposition (red staining signal) in tamoxifen-induced transgenic heart in response to ISO treatment. B, significantly reduced number of activated caspase 3–positive cardiomyocytes in tamoxifen-induced transgenic hearts with ISO treatment. C, significantly reduced level of cleaved PARP in tamoxifen-induced transgenic hearts with ISO treatment. The quantification of the Western blotting is in Fig. S2. D, well-preserved cardiac systolic function in tamoxifen-induced transgenic mice with ISO treatment. E, i.p. injection of rhBMP10 into WT mice (DBA/2J) reduced cardiac fibrosis in response to ISO treatment compared with saline-injected control mice in response to ISO treatment. F, i.p. injection of rhBMP10 into WT mice (DBA/2J) significantly preserved cardiac systolic function in response to ISO treatment. EF%, ejection fraction; FS%, fractional shortening.