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Impaired peptide sensitivity of 5T4-reactive CD4+ T-cell clones. Soluble IFN-γ, TNFα, and MIP-1β release by three 5T4-responsive CD4+ T-cell clones and a representative influenza A virus hemagglutinin (IAV-HA)-responsive CD4+ T-cell clone. Cytokine/chemokine measured by ELISA in response to overnight co-culture with T2-DR1–presenting cells and titrated cognate peptide. Representative examples (mean ± S.D.) of two independent experiments were performed in at least duplicate is shown.
