Figure 6.

Effect of Hfq on the accumulation of rbn mRNA and RNase BN. Overnight cultures were diluted into 100 ml of YT medium, and cells were harvested at the indicated time points. Total RNA or protein was prepared as described under “Experimental procedures” and subjected to RT-PCR or immunoblotting analysis. A, accumulation of rbn mRNA in WT and Δhfq strains. cDNA was synthesized using 1.2 μg of total RNA for RT-PCR. Equal amounts of total RNA were added to each lane. B, accumulation of RNase BN in WT and Δhfq strains. Twenty μg of protein was separated by 10% PAGE and subjected to immunoblotting analysis. Coomassie Blue staining was used for the loading control. A representative experiment carried out twice with essentially identical results is shown. The relative amount of rbn mRNA or RNase BN protein is indicated with an average ± S.D. from the two biological replicates by comparing to the 2-h sample, which was set at 100.