Figure 1.

Chemical genetic screening for inhibitors of activated ethylene-response mutants. A, the screening strategy scheme. For the screen, 2000 small molecules from the Microsource Spectrum Chemical Library were used to identify compounds (50–100 μm) that could rescue the root elongation defects of ctr1–1 and eto1–2. B, the structure of ponalrestat. C, the root lengths of 3-day-old Col-0, eto1–2, and ctr1–1 seedlings treated with ponalrestat or DMSO as the mock control. The seeds were germinated and grown on 1/2 MS medium supplemented with ponalrestat or DMSO. Bars represent mean ± S.D. of at least 10 seedlings; a Student's t test was used to compare ponalrestat-treated and mock-treated seedlings (***, p < 0.001). The experiment was repeated for at least three times with similar results. D, representative seedlings of the genotypes in (C). Scale bar = 1 mm.