Table 2.

Details of primers used in this study

gene	Primers	Sequences (5′ → 3′)	Tm	Amplicon (bp)	Reference
18S rRNA	Piro1-S	F1: CTTGACGGTAGGGTATTGGC	55 °C	1400	[27]
	Piro3-AS	R1: CCTTCCTTTAAGTGATAAGGTTCAC			[27]
	Piro-A	F2: ATTACCCAATMCBGACACVGKG	55 °C	407	[27]
	Piro-B	R2: TTAAATACGAATGCCCCCAAC			[27]
	P1	F: AACCTGGTTGATCCTGCCAGTAGTCAT	54 °C	1700	[20]
	P2	R:GATCCTTCTGCAGGTTCACCTAC			[20]
ITS region	ITS-F	F: GAGAAGTCGTAACAAGGTTTCCG	54 °C	1100	[20]
	ITS-2	R: ACAATTTGCGTTCAATCCCA			[20]