Fig. 5.

GmWRKY40 regulates soybean defense against P. sojae infection. a GmWRKY40 silenced hairy roots were hyper-susceptible to P. sojae infection. Hairy roots were inoculated with zoospore suspension (10⁴ zoospore/mL) of the P. sojae P6497R. P. sojae infection was monitored using a fluorescent microscope at 24, 36 and 48 hpi. Bar equals 0.5 mm. b Infection progress of P. sojae in soybean roots expressing EV or RNAi-GmWRKY40. The number of roots allowing hyphae penetration or oospore development were counted at 24, 36 and 48 hpi. This experiment were repeated three times with similar results