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. 2019 Dec 30;16:166. doi: 10.1186/s12985-019-1264-z

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Performance of CA16 LFS RT-RAA assay. a Optimization experiment of LFS RT-RAA reaction time. When the reaction time was longer than 20 min, the test line was visible. b Analytical sensitivity of the LFS RT-RAA assay. Lane1, 1 × 10⁶ copies, Lane2,1 × 10⁵copies, Lane3, 1 × 10⁴copies, Lane4, 1 × 10³copies, Lane5, 1 × 10² copies, Lane6, 1 × 10¹ copies, Lane7, 1copy, Lane8, no template control. c Analytical specificity of the LFS RT-RAA assay. Only CA16 specimen was amplified. The other samples were not amplified. Lane1, CA16, Lane2, EV71, Lane3, CA6, Lane4, CA10, Lane5, CA5, Lane6, CA9, Lane7, CA24, Lane8, CB2, Lane9, CB4, Lane10, PV2, Lane11,PV3, Lane12, Eco30, Lane13, HEV14, Lane14, no template control