Fig. 5.
Tumors with a higher tumor-to-stroma ratio (T:S) have increased CD16 expression on infiltrating monocytes (CD14+), and mature APCs (CD11c+HLA-DRhigh) expressing higher levels of PD-L1 and lower levels of CLEC9a. A: Histogram showing representative CD16 staining on monocytes (red) compared to FMO (grey). B: Differences in normalized CD16 expression compared to FMO on monocytes from tumors with low (T:S < 1.5; blue) or high (T:S > 1.5; orange) T:S. C: Flow plot showing representative gating of mature APCs. D, E: Mature APCs were stained for activating (ICOSL, B7-H3, CD40, CD86, CLEC9a) and inhibitory (B7-H4, B7-H3, PD-L1, PD-L2) surface molecules. Histograms show representative staining (red) compared to FMO (grey) (D). E: Normalized surface molecule expression on mature APCs from tumors with low (T:S < 1.5; blue) or high (T:S > 1.5; orange) T:S. Statistical significance was determined by Mann Whitney U test