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ESI and IR-MALDESI mass spectra of myoglobin in 70:30 (v/v) 5 mM ammonium acetate/ methanol (a and b), and 1 mM formic acid in 50:50 (v/v) water/methanol (c and d), respectively. Inserts: expanded spectra of m/z 2180-2230 show sodiated holo-Mb ions in charge state 8+.
