Table 2.
Mean IE values (eV) with 95% confidence intervals (CI) and full-width at half maximum (FWHM, eV) of BP cations for ESI and IR-MALDESI determined at IS-CID of 10-25 eV.
| Source | ESI | IR-MALDESI | |||||||
|---|---|---|---|---|---|---|---|---|---|
| Sample Type | Solution | Droplet | Sprayed Slide | ||||||
| IS-CID (eV) | R2 | Mean IE [CI] | FWHM | R2 | Mean IE [CI] | FWHM | R2 | Mean IE [CI] | FWHM |
| 10 | 0.998 | 1.73 [1.73, 1.73] | 0.38 | 0.995 | 1.68 [1.68, 1.69] | 0.33 | 0.988 | 1.74 [1.73, 1.75] | 0.39 |
| 15 | 0.998 | 1.91 [1.91, 1.91] | 0.48 | 0.984 | 1.85 [1.85, 1.87] | 0.50 | 0.983 | 1.86 [1.85, 1.87] | 0.44 |
| 20 | 0.996 | 2.13 [2.13, 2.13] | 0.59 | 0.987 | 2.05 [2.04, 2.06] | 0.61 | 0.971 | 1.97 [1.96, 1.99] | 0.59 |
| 25 | 0.999 | 2.33 [2.33, 2.33] | 0.56 | 0.990 | 2.29 [2.28, 2.30] | 0.64 | 0.962 | 2.11 [2.09, 2.13] | 0.74 |
| Source | IR-MALDESI | |||||
|---|---|---|---|---|---|---|
| Sample Type | Sprayed Slide (1 Laser Pulse) | Sprayed Slide underneath A Tissue Section | ||||
| IS-CID (eV) | R2 | Mean IE [CI] | FWHM | R2 | Mean IE [CI] | FWHM |
| 10 | 0.998 | 1.74 [1.74, 1.75] | 0.39 | 0.994 | 1.70 [1.69, 1.70] | 0.27 |
| 15 | 0.996 | 1.88 [1.87, 1.88] | 0.47 | 0.986 | 1.76 [1.75, 1.77] | 0.33 |
| 20 | 0.991 | 2.05 [2.04, 2.06] | 0.59 | 0.981 | 1.85 [1.84, 1.86] | 0.39 |
| 25 | 0.983 | 2.22 [2.20, 2.23] | 0.73 | 0.953 | 1.93 [1.91, 1.95] | 0.68 |
*
R2 stands for the regression coefficient of the sigmoidal fit.