Fig. 2.

Circ_0001667 inhibition decreased breast cancer cell proliferation and metastasis. a Circ_0001667 expression in MDA-MB-468 and BT549 cells. MDA-MB-468 and BT549 cells were infected with lentivirus with circ_0001667 shRNA for 48 h and total RNA was extracted for real time RT-PCR. b The survival ability in MDA-MB-468 cells with circ_0001667 down-regulation. MDA-MB-468 cells were infected with lentivirus with shRNA and the cell proliferation was assayed by CCK8 from day 0 to day 5. c The survival ability in BT549 cells with circ_0001667 down-regulation. MDA-MB-468 cells were infected with lentivirus with shRNA and the cell proliferation was assayed by CCK8 from day 0 to day 5. d The migration ability in MDA-MB-468 cells with circ_0001667 down-regulation. MDA-MB-468 and BT549 cells were infected with lentivirus with shRNA and the cell migration was assayed by transwell system. e The invasion ability in MDA-MB-468 cells with circ_0001667 down-regulation. MDA-MB-468 and BT549 cells were infected with lentivirus with shRNA and the cell migration was assayed by transwell chamber with Matrigel treatment. **p < 0.01