Fig. 5.

Circ_0001667/miR-125a-5p/TAZ activated Hippo pathway in breast cancer cells. a TAZ was a predicted target gene of miR-125a-5p by Targetscan. b TAZ 3′UTR activity in MDA-MB-468 cells with miR-125a-5p transfection. c TAZ mRNA in MDA-MB-468 and BT549 cells. Cells were transfected with miR-125a-5p TAZ mRNA was analyzed by qRT-PCR. d TAZ protein levels in MDA-MB-468 and BT549 cells. Cells were transfected with miR-125a-5p and the expression of TAZ was determined by western blotting. e Circ_0001667 promoted YAP, SAV1, TAZ expression. MDA-MB-468 and BT549 cells were transfected with Circ_0001667 shRNA or miR-125a-5p and the expression of YAP, SAV1, TAZ was determined by western blotting. f Cell proliferation in MDA-MB-468 and BT549 cells. Cells were transfected with circ_0001667 shRNA, miR-125a-5p or TAZ and cell survival ability was assayed by CCK8. g Cell invasion in MDA-MB-468 and BT549 cells. Cells were transfected with circ_0001667 shRNA, miR-125a-5p or TAZ and cell invasion ability was assayed by transwell system. **p < 0.01