Fig. 3.

Neoantigen-reactivity is contained within CD39⁺CD103⁺ CD8⁺ T cell subsets. a Flow cytometric sorting procedure adopted for the isolation of CD8⁺ T cell subsets according to CD39 and CD103 expression. Numbers within the gates represent the percentage of CD8⁺ cells contained in each subset. b Neoantigen-specific responses of the different T cell subsets upon co-culture with neo-epitopes. Peptide numbers are included for responses that were determined to be positive, and were taken along in the validation experiment. c IFN-γ production of the CD39⁺CD103⁺ CD8⁺ T cells upon co-culture with mutant (black) and corresponding wild type (gray) peptides, and a DMSO control (dashed), at different peptide concentrations. The mean ± standard deviation of the biological duplicates in the same experiment are depicted. d Flow cytometric analysis of the percentage of CD137⁺ T cells, depicted in the gates, within the CD8⁺ population of the expanded TIL upon co-culture with the mutant or wild type peptide, or DMSO control.