Figure 2.

Hypercapnia causes a proteomic signature suggesting depressed ribosomal function and structure. (A) Covariables chosen a priori were investigated to determine their possible confounding effect on ribosomal RNA expression and puromycin incorporation. Extensor digitorum longus muscles were sampled and submitted for a large-scale proteomic analysis. (B) Volcano plot highlighting proteins found to be downregulated (green circles) and upregulated (red circles). Eif4e3 and Eif3m are indicated by arrows and significantly downregulated (P < 0.001). (C) Ontological enrichment terms identified as significant or nearly significant in this study. n = 6 (3 normocapnia and 3 hypercapnia). Eif3m = eukaryotic translation initiation factor 3 subunit m; Eif4e3 = eukaryotic translation initiation factor 4E family member 3; GO = gene ontology; WT = wild type.