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. 2020 Jan 1;201(1):63–72. doi: 10.1164/rccm.201906-1113OC

Figure 1.

Figure 1.

Flow cytometric identification of innate lymphocyte subsets in human lung tissue allograft biopsies. Shown is a flow cytometric analysis of a representative lung allograft biopsy. Total events were gated by (A) forward and side scatter gating (FSC and SSC), (B) doublet discrimination for single cells, (C) live cells, and (D) hematopoietic cells (CD45). (E) A combination of lineage marker antibodies (CD3, CD5, Cd19 FcεR1, CD11b, CD11c, CD14, and CD16) identified the lineage negative fraction (pink box). (F) Natural killer (NK) cells were identified as CD56+CD127− (purple box). (F) Total innate lymphoid cells (ILCs) were identified as lineage negative CD127+ (blue box) and then split into ILC1, ILC2, and ILC3 subsets. (G) ILC2s were defined as CRTH2+ (green box). (H) ILC1s were defined as CRTH2−CD117+NKp44+/− (red box). (H) ILC3s were defined as CRTH2−CD117+NKp44+/− (teal box). Data are representative of n = 18 lung transplant recipient patients (36 total samples; 1 prereperfusion and 1 postreperfusion allograft biopsy taken for each lung transplant recipient).