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. 2020 Jan 1;201(1):63–72. doi: 10.1164/rccm.201906-1113OC

Figure 2.

Figure 2.

Single-cell resolution of innate immune cell subsets in pre- and postreperfusion lung allograft biopsies in transplant patients. (A–F) Representative flow cytometric gating of innate lymphoid cell (ILC) subsets from lung allograft biopsies taken before or after reperfusion from patients diagnosed with interstitial lung disease (ILD) (A–C) or chronic obstructive pulmonary disease (COPD) (D–F). (A and D) Natural killer (NK) cells were identified as CD56+CD127− (purple box). (A and D) Total ILCs were identified as lineage negative CD127+ (blue box), and then split into ILC1, ILC2, and ILC3 subsets. (B and E) ILC2s were defined as CRTH2+ (green box). (C and F) ILC1s were defined as CRTH2−CD117+NKp44+/− (red box). (C and F) ILC3s were defined as CRTH2−CD117+NKp44+/− (teal box). Data are representative of one prereperfusion and one postreperfusion allograft biopsy taken for each lung transplant recipient; n = 15 recipients. N/D = not detected.