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. 2020 Jan 1;201(1):63–72. doi: 10.1164/rccm.201906-1113OC

Figure 4.

Figure 4.

Distribution of multiple innate lymphoid cell (ILC) subsets fluctuates after donor graft reperfusion. (A–C) Flow cytometric analysis of total ILC frequencies (live, CD45+ lineage negative [Lin−] CD127−) in pre- and postreperfusion allograft biopsies (A; n = 15) from lung transplant recipients with interstitial lung disease (ILD) (B; n = 6) or chronic obstructive pulmonary disease (COPD) (C; n = 8). (D–F) Flow cytometric analysis of total ILC2 frequencies (live, CD45+Lin−CD127−CD56−CRTH2+) in pre- and postreperfusion allograft biopsies (D; n = 8) from ILD (E; n = 4) or COPD (F; n = 3) lung transplant recipients. (G–I) Flow cytometric analysis of total natural killer (NK) p44+ILC1 frequencies (live, CD45+Lin−CD127−CRTH2−CD56+/−CD117−NKp44+) in pre- and postreperfusion allograft biopsies (G; n = 14) from lung transplant recipients with ILD (H; n = 6) or COPD (I; n = 5). (J–L) Flow cytometric analysis of total NKp44−ILC1 frequencies (live, CD45+Lin−CD127−CRTH2−CD56+/−CD117−NKp44−) in pre- and postreperfusion allograft biopsies (J; n = 10) from lung transplant recipients with ILD (K; n = 6) or COPD (L; n = 4). (M–O) Flow cytometric analysis of total NKp44+ILC3 frequencies (live, CD45+Lin−CD127−CRTH2−CD56+/−CD117+NKp44+) in pre- and postreperfusion allograft biopsies (M; n = 9) from lung transplant recipients with ILD (N; n = 5) or COPD (O; n = 3). (P–R) Flow cytometric analysis of total NKp44−ILC3 frequencies (live, CD45+Lin−CD127−CRTH2−CD56+/−CD117+NKp44−) in pre- and postreperfusion allograft biopsies (P; n = 8) from lung transplant recipients with ILD (Q; n = 3) or COPD (R; n = 4). NCR = natural cytotoxicity receptor.