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. 2018 Aug 25;68(10):1718–1724. doi: 10.1093/cid/ciy728

Figure 3.

Figure 3.

Vaccination of female BALB/cJ mice with rPbSEA-1A protects their pups from challenge with P. berghei ANKA. Antibody responses of rPbSEA-1A assayed by bead-based endpoint titration ELISA in vaccinated female mice and their offspring in the (A) first, (D) second, and (G) third trials. The bars represent mean fluorescence; the error bars represent SEMs. Black bars represent anti–rPbSEA-1A IgG responses in female adult mice immunized with rPbSEA-1A or adjuvant alone. The white bars represent anti–rPbSEA-1A IgG responses in their pups. (B) In the first trial, pups born to control dams (Control Litters #1 and #2, total n = 10) had 8.4-fold higher parasitemia on day 9 post-challenge compared to pups born to the PbSEA-1A vaccinated dam (n = 5), who produced high-titer anti–PbSEA-1 antibodies (PbSEA-1 Litter #1, P < .002). Error bars represent SEMs. (C) In the first trial, BALB/cJ pups born to the vaccinated dam who produced high-titer anti–PbSEA-1A (PbSEA-1 Litter #1, n = 5) had 1.67-fold longer median survival times compared to pups born to control dams (Control Litters #1 and #2, total n = 10, P < .001). (E) In the second trial, BALB/cJ pups born to the control dam (Control Litter, n = 4) had 4.8-fold higher parasitemia on day 9 post-challenge (and a 170-fold higher parasitemia on day 17, the day of maximal parasitemia difference) compared to pups born to the 2 PbSEA-1A–vaccinated dams (PbSEA-1 Litters #1 and #2, n = 10, P < .005). Error bars represent SEMs. (F) In the second trial, BALB/cJ pups born to the vaccinated dams had 1.36-fold longer median survival times compared to pups born to the control dam (P < .003). (H) In the third trial, BALB/cJ pups born to the control dam had 27-fold higher parasitemia on day 9 post-challenge compared to pups born to the PbSEA-1A–vaccinated dam (P < .0001). Error bars represent SEMs. (I) In the third trial, BALB/cJ pups born to the vaccinated dam had 1.47-fold longer median survival times compared to pups born to the control dam (P < .001). Abbreviations: IgG, immunoglobulin G; ELISA, enzyme linked immunosorbent assay; PbSEA-1, Plasmodium berghei Schizont Egress Antigen-1; PfSEA-1, Plasmodium falciparum Schizont Egress Antigen-1; SEM, standard error of the mean.