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. Author manuscript; available in PMC: 2020 Oct 14.
Published in final edited form as: ACS Comb Sci. 2019 Sep 6;21(10):650–655. doi: 10.1021/acscombsci.9b00113

Figure 1.

Figure 1.

Assay reagent and workflow schema of the solution-phase DNA compatibility assessment platform (A) The NH2-HPT substrate 1 contains a primary amine for on-DNA synthesis displayed from the covalent DNA duplex headpiece, hp-DNA. A ClaI restriction endonuclease recognition sequence (teal) separates the hp-DNA from the forward primer binding site (blue nucleobases) and ligation junction. (B) NH2HPT substrate 1 is used in on-DNA chemical synthesis to couple monomer 2. Crude reaction mixture is ligated to a double-stranded oligonucleotide containing the reverse primer binding site (dsR) to furnish templates for PCR amplification.