Antibodies |
|
|
Mouse monoclonal anti-FASN |
BD Biosciences |
Cat# 610962; RRID:AB_398275 |
Rabbit monoclonal anti-CPT1A |
Cell Signaling Technology |
Cat#12252 |
Goat polyclonal anti-CD36 |
R&D Systems |
Cat# AF1955; RRID:AB_355073 |
Rabbit polyclonal anti-CD36 |
Novus Biologicals |
Cat#NB400–144; RRID:AB_10003498 |
Rabbit polyclonal anti-β-Actin |
Santa Cruz Biotechnology |
Cat# SC1616-R; RRID:AB_630836 |
Rabbit monoclonal anti-HER2 |
Cell Signaling Technology |
Cat# 2165; RRID:AB_560966 |
Rabbit polyclonal anti-p-HER2 (Tyr1221/1222) |
Cell Signaling Technology |
Cat# 2249S; RRID:AB_2099241 |
Rabbit monoclonal anti-pAKT (Thr308) |
Cell Signaling Technology |
Cat# 2965; RRID:AB_2255933 |
Rabbit polyclonal anti-AKT |
Cell Signaling Technology |
Cat# 9272; RRID:AB_329827 |
Rabbit monoclonal anti-pERK1/2 (Thr202/Tyr204) |
Cell Signaling Technology |
Cat# 4370; RRID:AB_2315112 |
Rabbit monoclonal anti-ERK |
Cell Signaling Technology |
Cat#4695; RRID:AB_390779 |
Mouse monoclonal anti-CD36, FITC |
eBioscience |
Cat# 11–0369-41; RRID:AB_10719431 |
Mouse monoclonal anti-CD36 (Clone JC63.1; Function Blocking) |
Cayman Chemical |
Cat# 188150; RRID:AB_10077812 |
Mouse monoclonal mouse IgA isotype control |
Abcam |
Cat# ab37322 |
Chemicals, Peptides, and Recombinant Proteins |
|
|
Oil Red O |
Cayman Chemical |
Cat# 14419 |
BODIPY 493/503 (4,4-Difluoro-1,3,5,7,8-Pentamethyl-4-Bora-3a,4a- Diaza-s-Indacene) |
Invitrogen |
Cat# D3922 |
BODIPY FL C16 (4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s- Indacene-3-Hexadecanoic Acid) |
Invitrogen |
Cat# D3821 |
[1-14C]-Acetic Acid, sodium salt |
PerkinElmer |
Cat# NEC084H001MC |
Annexin V Alexa Fluor® 647 conjugate |
Invitrogen |
Cat# A23204 |
(−)-trans-C75 |
Cayman Chemical |
Cat# 9000784 |
Sulfosuccinimidyl Oleate (SSO) |
Cayman Chemical |
Cat# 11211 |
Etomoxir (sodium salt) |
Cayman Chemical |
Cat# 11969 |
Lapatinib |
LC Laboratories |
Cat# 388082–77-7 |
SV Total RNA Isolation System |
Promega |
Cat# Z3105 |
qScript cDNA synthesis kit |
Quanta |
Cat# 95047–100 |
iTaq Universal SYBR® Green Supermix |
Bio-Rad |
Cat# 1725124 |
Lipofectamine RNAiMAX Transfection Reagent |
Invitrogen |
Cat# 13778150 |
Critical Commercial Assays |
|
|
HumanHT-12 v4 BeadChip |
Illumina |
Cat# BD-103–0204 |
CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) |
Promega |
Cat# G3582 |
Seahorse XF Mito Stress Test Kit |
Agilent |
Cat# 103015–100 |
Seahorse XF Glycolysis Stress Test Kit |
Agilent |
Cat# 103020–100 |
Matrigel Matrix |
Corning |
Cat# 354234 |
Deposited Data |
|
|
Microarray data |
This paper |
GSE136304 |
RNaseq data |
Fumagalli et al., 2017 |
N/A |
Experimental Models: Cell Lines |
|
|
Human: BT474 |
ATCC |
RRID:CVCL_0179 |
Human: rBT474 |
Kurokawa et al., 2013 |
N/A |
Human: SKBR3 |
ATCC |
RRID:CVCL_0033 |
Human: rSKBR3 |
Kurokawa et al., 2013 |
N/A |
Human HCC202 |
ATCC |
RRID:CVCL_2062 |
Human HCC202R |
This paper |
N/A |
Experimental Models: Organisms/Strains |
|
|
Mouse: FVB-Tg(MMTV-Erbb2)NK1Mul/J |
The Jackson Laboratory |
RRID:IMSR_JAX:005038 |
Mouse: Tg(MMTV-cre)4Mam/J |
The Jackson Laboratory |
RRID:IMSR_JAX:003553 |
Mouse: CD36F/F
|
Maria Febbraio |
N/A |
Mouse: NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ |
The Jackson Laboratory |
RRID:IMSR_JAX:005557 |
Oligonucleotides |
|
|
CD36 Human siRNA Oligo Duplex |
Origene |
Cat# SR319610 |
Trilencer-27 Universal scrambled negative control siRNA duplex |
Origene |
Cat# SR30004 |
qPCR Primer Sequences |
This Paper |
Table S3 |
Software and Algorithms |
|
|
GraphPad Prism v7 |
GraphPad |
https://www.graphpad.com/ |
R: A Language and Environment for Statistical Computing |
R Foundation for Statistical Computing |
https://www.r-project.org/ |
Molecular Signatures Database |
Broad Institute |
http://software.broadinstitute.org/gsea/msigdb/index.jsp |