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A lipid raft-targeted biosensor reveals distinct patterns of PKA activity in the leading edge during cell migration. SKOV-3 cells expressing LynAKAR4 were plated on fibronectin-coated glass coverslips and monitored via live-cell FRET microscopy 4 h after plating. Images were acquired every 2 min for 45 min (bar = 25 μm). Kymograph generated from linescan shown at the beginning of the video.
