Table 1. Sequence of RT-qPCR primers.
| Target Gene | Sequence |
|---|---|
| β2M |
(s) CAGCGTACTCCAAAGATTCAGG (as) AGTCAACTTCAATGTCGGATGG |
| GAPDH |
(s) TCTATAAATTGAGCCCGCAGC (as) CGCCCAATACGACCAAATCC |
| ICAM-1 |
(s) GTCCTGTATGGCCCCCGACT (as) GGGCAGTGGGAAAGTGCCAT |
| E-selectin |
(s) AGGTTCCTTCCTGCCAAGTGGT (as) TGTCCGAGCTGCAGAGCCAT |
| sXBP1 |
(s) GGCATTCTGGACAAGTTGG (as) TAGGCAGGAAGATGGCTTTGG |
| ATF4 |
(s) CATCTGTATGAGCCCTGAGTC (as) CGAGAACCACGAGGAACACC |
| ATF5 |
(s) AGGCTGGATCCTCAAAATCAC (as) CGGCGACACTCTTCCCTCTG |
| CHOP |
(s) CACTCTTGACCCTGCTTCTC (as) TCTGACTGGAATCTGGAGAG |
| GADD34 |
(s) GAAGAGGGAGTTGCTGAAGAGG (as) GGAGACAAGGCAGAAGTAGAGG |
| GRP78 |
(s) GAACGTCTGATTGGCGATGC (as) TCAACCACCTTGAACGGCAA |
β2M, β2-microglobulin; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; ICAM-1, intracellular adhesion molecule 1; sXBP1, X-box binding protein 1; ATF4, activating transcription factor 4; ATF5, activating transcription factor 5; CHOP, C/EBP homologous protein; GADD34, growth arrest and DNA-damage inducible-34; GRP78, glucose-regulated protein-78.