Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain Mus musculus (males and female) | C57BL6J | Janvier | ||
| Mus musculus (males and female) | ΔNp73CreIRESGFP | Tissir et al., 2009 | ΔNp73Cre | |
| Mus musculus (males and female) | Wnt3aCre | Yoshida et al., 2006 | Wnt3aCre | |
| Mus musculus (males and female) | TauloxP-stop-loxP-MARCKSeGFP-IRES-nlslacZ | Hippenmeyer et al., 2005 | TauGFP | |
| Mus musculus (males and female) | Baxtm2Sjk;Bak1tm1Thsn/J | Takeuchi et al., 2005 | Baxlox/lox | |
| Mus musculus (males and female) | ROSA26loxP-stop-loxP-Tomato | Madisen et al., 2010 | R26mT | |
| Mus musculus (males and female) | ROSA26 loxP-stop-loxP- Kcnj2-cherry/+ | Moreno-Juan et al., 2017 | R26Kir2.1/+ | |
| Mus musculus (males and female) | Ai32(RCL-ChR2(H134R)/EYFP | https://www.jax.org/strain/012569 | ChR2lox | |
| Antibody | rabbit polyclonal anti-DsRed | Takara | RRID:AB_10013483 | IF(1:500) |
| Antibody | mouse monoclonal anti-Reelin | Merck Millipore | RRID:AB_565117 | IF(1:300) |
| Antibody | mouse monoclonal anti-Gephyrin | Synaptic systems | RRID:AB_2619837 | IF(1:250) |
| Antibody | rabbit polyclonal anti-GAD65/67 | Merck | RRID: AB_22
78725 |
IF(1:250) |
| Antibody | guinea-pig anti-KCC2 | D Ng and S Morton TM Jessell’s lab |
IF(1:4000) | |
| Antibody | donkey anti-mouse Alexa-488 |
Jackson ImmunoResearch Laboratories | RRID:AB_2340846 | IF(1:800) |
| Antibody | donkey anti-rabbit Cy3 |
Jackson ImmunoResearch Laboratories | RRID:AB_2307443 | IF(1:800) |
| Antibody | donkey anti-rabbit Alexa-647 | Molecular Probes | RRID:AB_2536183 | IF(1:500) |
| Antibody | donkey anti-chick Alexa-488 | Jackson ImmunoResearch Laboratories | RRID:AB_2340375 | IF(1:1000) |
| Antibody | donkey anti-mouse Alexa-555 | Molecular Probes | RRID:AB_2536180 | IF(1:1000) |
| Antibody | goat anti-guinea pig Alexa-555 | Molecular Probes | RRID:AB_2535856 | IF(1:1000) |
| Antibody | DAPI (4', 6-diamidino-2-phenylindole) | Invitrogen Molecular Probes | RRID:AB_2629482 | IF(1:2000) |
| Antibody | DyLight 488 streptavidin | Vector Labs | SP-4488 | |
| Sequence-based reagent | CRE genotyping 188 f 167 r |
This paper | PCR primers |
188 f: TGA TGG ACA TGT TCA GGG ATC 167 r: GAA ATC AGT GCG TTC GAA CGC TAG A |
| Sequence-based reagent | R26Kir2.1/+genotyping AAY101 AAY103 SD297 |
PCR primers |
AAY101: AAAGTCGCTCTGAGTTGTTAT (Rosa26 forward WT) AAY103: GGGAGCGGGAGAAATGGATATG (Rosa26 reverse WT) SD297: GGCCATTTACCGTAAGTTATG (CAG promoter reverse) |
|
| Chemical compound, drug | Paraformaldehyde | Sigma-Aldrich | CAT:P6148 | |
| Chemical compound, drug | Triton 100X | Eurobio | CAT:GAUTTR00-07 | |
| Chemical compound, drug | SR95531 | Abcam | Ab120042 | |
| Chemical compound, drug | 4-AP | Sigma Aldrich | A-0152 | |
| Software, algorithm | IMARIS software 8.4. | IMARIS | RRID:SCR_007370 | |
| Software, algorithm | GraphPad Prism 7.0 | GraphPad Software |
RRID:SCR_000306 | |
| Software, algorithm | ImageJ/FIJI | NIH | RRID:SCR_002285 | |
| Software, algorithm | Adobe Photoshop CS6 |
Adobe Systems |
RRID:SCR_014199 | |
| Software, algorithm | pClamp10.1 | Molecular Devices | RRID:SCR_011323 | |
| Software, algorithm | IGOR Pro 6.0 | Wavemetrics | RRID:SCR_000325 | |
| Software, algorithm | NeuroMatic | Wavemetrics | RRID:SCR_004186 |