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. 2019 Dec 31;9:20416. doi: 10.1038/s41598-019-56882-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Culture-dependent analysis of the 16S rRNA gene sequences. (a) Neighbor‐joining phylogenetic tree of the isolated bacteria. Each color represents a phylum level of the bacterial community. The associated bacteria were isolated from KW, FBC2, MW, DC, BJ and HC samples. The number in parentheses indicates the number of isolated associated bacteria. Most bacteria belonged to the class Alpha-proteobacteria. GenBank accession numbers are provided in parentheses. (b) Pseudomonas species were the most frequently isolated species from the culturable bacteria.