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. 2019 Dec 31;9:20409. doi: 10.1038/s41598-019-56905-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The concentration–response relationship for the neuroprotective effects of nafamostat against NMDA-induced cell death in primary rat cortical neurons. Neurons were incubated for 2 h simultaneously with nafamostat and NMDA, and cell viability was assessed using MTS assays. Absorbance in each well was normalised to that in the untreated wells (Untreated; medium alone) and presented as percentages. Each value represents the mean ± S.E. of six replicates. ^***P < 0.001, compared with untreated control; ^†††P < 0.001, compared with NMDA alone by Aspin–Welch’s t-test. ^###P < 0.001, compared with NMDA alone by Dunnett’s multiple comparison test.