Pancreas sections from 8-week-old Glis3Δpanc mice were stained for PDX1 and MAFA (A) or GLUT2 (B). Proliferation was measured via Ki67 and PDX1 double staining of 2-week old Glis3Δpanc mice (C), and quantified (D). Apoptosis was measured via TUNEL and PDX1 double staining of 2-week old Glis3Δpanc mice (E), and quantified (F). Results from N=3 mice for each genotype, error bars = SEM, * indicates p<0.05. Arrows indicate MAFA+PDX1+ (A), GLUT2+PDX1+ (B), Ki67+PDX1+ (C), or TUNEL+PDX1+ (D) cells. Representative images are shown.