Skip to main content
. Author manuscript; available in PMC: 2020 Oct 1.
Published in final edited form as: Endocr Relat Cancer. 2019 Oct;26(10):765–778. doi: 10.1530/ERC-19-0262

Figure 1.

Figure 1.

PBK is overexpressed in ACC tumors and dictates survival outcomes. A. PBK mRNA expression in ACC (n=77) is 12 fold higher than normal adrenal tissues ( n=14) and 7 fold higher than adenomas ( n=22 ) in microarray gene expression studies. B,C. Kaplan Meier analysis of survival using Graph Pad Prism with normalized RNA seq data from the TCGA cohort of ACC (n=75) available in cbioportal. RNA expression was normalized using RSEM.10% of the population in TCGA cohort had a Z s core >2 and was designated to have PBK mRNA upregulation. High PBK expression associated with poor overall survival (OS) and poorer disease free survival (DFS) (** p< 0.01). D, E. PBK protein expression in ACC tumor tissues and ACC cell lines and PDXs. Quantification of E is shown on the right F. IHC with anti-PBK in normal adrenal show no nuclear staining but strong nuclear and diffused cytoplasmic staining in ACC.