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. Author manuscript; available in PMC: 2020 Sep 1.
Published in final edited form as: Endocr Relat Cancer. 2019 Sep;26(9):739–750. doi: 10.1530/ERC-19-0107

Figure 5.

Figure 5.

(A and B) The combined treatment of PLX and PD synergistically decreased the MYC mRNA expression in ATC cell lines. The mRNA level of the MYC gene was measured by real-time RT–PCR in THJ-11T cells (A) and THJ-16T cells (B). (C and D) The combined treatment of PLX and PD synergistically decreased the MYC mRNA expression in ATC xenograft tumors. Quantitative PCRs were performed using 200 ng of total RNA. GAPDH was used as a reference. (E and F) The combined treatment of PLX and PD synergistically reduced the recruitment of BRD4 on the promoter of the MYC gene in THJ-11T cells (E) and THJ-16T cells (F). ChIP assay was carried out using normal rabbit IgG or BRD4 antibody. The experiments were carried out in triplicates. The p values are shown (n=3).